We compared HbA
1c in 1,038 diabetic patients using 2 HPLC instruments, Hi-AUTO HA-8150 and Hi-AUTO HA-8160. HA-8150 cannot separate the #C peak from the HbA
1c peak, whereas HA-8160 can separate them. In 697 patients, the differences in HbA
1c were less than 0.3%. In 128 patients, HbA
1c measured with HA-8150 was higher than that measured with HA-8160 in 2 consecutive examinations. The greatest difference in HbA
1c was 2.5%. These patients had been clinically diagnosed with renal failure, hypothyroidism, and abnormal hemoglobinemia. In 22, HbA
1c measured with HA-8160 was higher than that measured with HA-8150 in 2 consecutive examinations. The greatest difference in HbA
1c was 1.4%. We found that HbF was high for most of these patients.
View full abstract