All issues
Volume 44, Issue 2
Displaying 1-11 of 11 articles from this issue
- |<
- <
- 1
- >
- >|
-
Masahiko Kawasumi, Masako Mitsumata, Satoshi Shimada, Tomoyuki Arisaka ...2001 Volume 44 Issue 2 Pages 115-120
Published: February 28, 2001
Released on J-STAGE: March 02, 2011
JOURNAL FREE ACCESSVascular endothelial dysfunction in diabetic patients is suspected of being induced by advanced glycation end products (AGEs). Recent studies have indicated that heparan sulfate proteoglycan, which is a major component of the glycosaminoglycans (GAGs) synthesized by endothelial cells (ECs), regulates anticoagulant activity by binding with antithrombin III. We therefore examined the effects of AGEs on GAG synthesis and DNA synthesis by ECs. After incubating porcine aortic ECs in serum-free medium containing AGEmodified bovine serum albumin (AGE-BSA), synthesis of GAGs and DNA was assayed on the basis of [35S]-sulfate and [3H]-thymidine incorporation, respectively. Both the synthesis and secretion of GAGs by ECs were significantly reduced after AGE-BSA treatment. After ECs were exposed to 1 mg/ml AGE-BSA for 55 h, GAG synthesis was 86% of that in cells incubated with unmodified BSA (p<0.05), and it declined to 75% at 73 h (p<0.01). After exposure to 0.5 mg/ml AGE-BSA for 78h, GAG synthesis was 87% of the control level (p<0.05), and it fell to 84% in the presence of 1mg/ml AGE-BSA (p<0.05). Treatment with AGE-BSA for 48-60h also reduced [3H]-thymidine incorporation by ECs to 68-44% of the control level (p<0.01). The changes induced by AGE-BSA treatment were inhibited by an antioxidant (probucol). These results suggest that AGEs suppress GAG and DNA synthesis by ECs through oxidative stress and induce endothelial dysfunction by inhibiting endothelial anticoagulant activity.View full abstractDownload PDF (1190K) -
Akane Katagiri, Shuji Hashimoto, Yasuo Ohashi, Nobuaki Kuzuya, Yasunor ...2001 Volume 44 Issue 2 Pages 121-126
Published: February 28, 2001
Released on J-STAGE: March 02, 2011
JOURNAL FREE ACCESSWe prospectively evaluated the effect of an educational videotape on diabetes produced by the Japan Diabetes Foundation in 11 courses provided by health centers and/or local administrations in the Kanto eastern region of Japan. Some 281 participants were devided into 2; one with a videotape player randomly allocated to either the videogroup or controls and one having no videotape player assigned to controls. The 138 videogroup participants were asked to watch the video at home. Baseline data, including the diabetes knowledge score, relative energy intake and HbA1c, were collected on the first day of the course. The diabetes knowledge score was assessed again on the last day of the course, and relative energy intake and HbA1c examined 2 months after the course. Of 198 subjects, 98 in the videogroup and 102 controls completed the second knowledge and energy intake assessment.
The videogroup showed a significantly greater increase in the diabetes knowledge score than controls (+6.0 versus+4.6, p=0.031), but results in net change of relative energy intake and HbA1c did not differ statistically significantly.View full abstractDownload PDF (1016K) -
Masako Ozaki, Hironori Yamasaki, Yoshihiko Yamaguchi, Hideaki Kondo, N ...2001 Volume 44 Issue 2 Pages 127-133
Published: February 28, 2001
Released on J-STAGE: March 02, 2011
JOURNAL FREE ACCESSGlutathione (GSH) is involved in deoxidation and elimination of hydrogen peroxide and other reactive oxygen species, and plays an important role in the antioxidant system. To investigate the effect of GSH on insulin gene expression, we established insulin secreting cells MIN 6 that stably transfected with the ribozyme against γ-glutamylcysteine synthetase (γ-GCS) an enzyme limiting GSH synthesis. Resultant cell lines exhibited suppressed intracellular GSH synthesis. We transiently transfected luciferase expression vector driven by preproinsulin gene promoter region-1998 to+237 to ribozyme transfected MIN 6 cells. Luciferase activity increased about 15 and 5 times in ribozyme transfected MIN 6 cells compared to untransfected MIN 6 cells and MOCK. Transfection with deletion constructs (-879 to+273) wasshown 30% attenuation of insulin promoter activity in ribozyme transfected MIN 6 cells compared to that seen with the full promoter length, while activity was preserved in untransfected MIN 6 cells and MOCK. These results suggest that lowering of intracellular GSH may in part regulate insulin gene expression in insulin secreting cells.View full abstractDownload PDF (998K) -
Tomio Kametani2001 Volume 44 Issue 2 Pages 135-140
Published: February 28, 2001
Released on J-STAGE: March 02, 2011
JOURNAL FREE ACCESSTo elucidate risk factors for hypertension, the relationships among the incidences of hypertension, diabetes mellitus, obesity and hypertriglyceridemia were examined in the4, 114 Japanese subjects (1, 978men and2, 136 women).Significant positive correlations among blood pressure, body mass index, plasma glucose and triglyceride levels were identified.Subjects with diabetes mellitus had a 1.59-fold (95%CI1.08-2.34) higher incidence of hypertension compared with that of normal subjects.Also, obese subjects had a1.85-fold (1.47-2.34) higher incidence of hypertension compared with that of normal subjects. Subjects with hypertriglyceridemia had a1.41-fold (1.15-1.72) higher incidence of hypertension compared with that of normal subjects.The combination of diabetes mellitus, obesity and hypertriglyceridemia had a5.14-fold (2.67-9.92) higher incidence of hypertension compared with normal subjects.
These findings demonstrate that abnormalities in glucose tolerance, obesity and hypertriglyceridemia are independent factors of hypertension.View full abstractDownload PDF (871K) -
Yoshihiro Ikezawa, Kyuzi Kamoi, Masato Takagi, Kenzo Kaneko, Hideo Sas ...2001 Volume 44 Issue 2 Pages 141-146
Published: February 28, 2001
Released on J-STAGE: March 02, 2011
JOURNAL FREE ACCESSThe aim of this study was to evaluate the prevalence and clinical significance of antibodies to glutamic acid decarboxylase (anti-GAD) in 103 diabetic patients with secondary failure of sulfonylurea agents (SU-F).
The percentage of anti-GAD positive cases among the SU-F patients was 28.2%.
The mean interval (8.9 years) between the discovery of diabetes and the institution of insulin therapy in the patients with anti-GAD antibody was shorter (p<0.05) than in the patients without anti-GAD antibody (12.1 years). The mean body mass index (21.1 kg/m2) of the anti-GAD-positive patients was lower (p<0.05) than that of the anti-GAD-negative patients (23.1 kg/m2). The mean urinary C-peptide excretion of the anti-GADpositive patients was lower than that of the anti-GAD-negative patients (29 rig/day vs. 65 fig/day, p<0.05), and the insulin dose then required was higher (0.67 U/kg/day vs.0.37 U/kg/day, p<0.05).
In conclusion, nearly 30% of DM-SF patients were anti-GAD-antibody-positive, suggesting that an autoimmune mechanism may play an important role in the pathogenesis in some diabetic patients with secondary failure of sulfonylurea agents. Patients who appear to have type 2 diabetes should be examined carefully by determining whether they are positive for autoantibody to GAD.View full abstractDownload PDF (879K) -
Motoi Sohmiya, Toshiaki Mori, Kunio Koshimura, Yuzuru Kato2001 Volume 44 Issue 2 Pages 147-152
Published: February 28, 2001
Released on J-STAGE: March 02, 2011
JOURNAL FREE ACCESSA 55-year-old man was diagnosed with diabetes mellitus three years after the onset of SBMA. He had a family history of SBMA in a grandfather. Androgen receptor gene analysis revealed an increase in number of CAG repeats to 47. In spite of appropriate diet and exercise, periodic changes in HbA1c along with serum CK (MM type) and serum GPT occurred for 2 years. Close correlations were observed among the changes in serum CK, GPT, and HbA1c values during the clinical course. The HbA1c levels were positively correlated with the serum CK (r=0.535, p=0.0103), serum GPT levels (r= 0.475, p= 0.0294), and serum aldolase levels (r= 0.795, p= 0.0326). SBMA is an adult-onset neuron disease often accompanied by diabetes mellitus. However, the relationship between SBMA and diabetes mellitus has not been fully elucidated. Our findings suggest that muscular damage affected contorol of the diabetes mellitus in the patient.View full abstractDownload PDF (652K) -
[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...2001 Volume 44 Issue 2 Pages 153-156
Published: February 28, 2001
Released on J-STAGE: March 02, 2011
JOURNAL FREE ACCESS -
[in Japanese], [in Japanese], [in Japanese], [in Japanese]2001 Volume 44 Issue 2 Pages 157-160
Published: February 28, 2001
Released on J-STAGE: March 02, 2011
JOURNAL FREE ACCESS -
[in Japanese]2001 Volume 44 Issue 2 Pages 161-162
Published: February 28, 2001
Released on J-STAGE: March 02, 2011
JOURNAL FREE ACCESS -
Makoto Tominaga, Isao Kobayashi, Katsuhiko Kuwa, Izumi Takei, Tadao Ho ...2001 Volume 44 Issue 2 Pages 165-176
Published: February 28, 2001
Released on J-STAGE: March 02, 2011
JOURNAL FREE ACCESSTo promote standardization and the proper use of 6 devices now available in Japan for self-monitoring blood glucose (SMBG), the Japan Diabetes Society (JDS) Committee on Standardization of Laboratory Testing Related to Diabetes Mellitus conducted surveys in cooperation with committees of the Japan Society of Clinical Chemistry (JSCC) and the Japan Society of Clinical Laboratory Automation (JSCLA) Surveys also targeted confirmation that these devices would meet international requirements for SMBG prepared by the ISO/TC 212 Committee. Surveys consisted of 3 parts:(1) questionnaires submitted to 6 SMBGdevice-manufacturers, (2) experiments using patient blood samples to evaluate actual device-to-device variation, and (3) questionnaires provided to trustees of the Japan Diabetes Society. Results from questionnaires on SMBG devices manufacturers showed these devices met the ISO/TC 212 requirements, and that all converted measured blood glucose to different data shown on devices displays. Results from experiments showed significant device-to-device variation, with devices falling into 3 types:(1) 2 that indicated values close to adjusted earlobe plasma glucose measured with the reference hexokinase method, (2) 1 that in dicated values close to venous plasma glucose measured by hexokinase method, and (3) 3 that indicated values falling between (1) and (2). Results from questionnaires answered by Japan Diabetes Society trustee showed that they disagreed about data conversion in devices for SMBG. With the committee finding itdifficult to propose recommendations on the standardization of devices for SMBG, it will continue discussions on standardizing reference and adjustment methods, to reduce device-to-device variation. Information on different features of devices for SMBG should be disseminated nationwide to health care professionals such as physicians nurses in charge of teaching, instruction, and management of devices for SMBG and to patients as users. Responsibility must be taken by the Japan Diabetes Society for publication of a resource guide similar to that published annually in the United States by the American Diabetes Association.View full abstractDownload PDF (1426K) -
2001 Volume 44 Issue 2 Pages 179-188
Published: February 28, 2001
Released on J-STAGE: March 02, 2011
JOURNAL FREE ACCESSDownload PDF (2331K)
- |<
- <
- 1
- >
- >|