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E. coli KG 980,a vitamin B_6 auxotroph, could not utilize for its growth three phosphate forms of vitamin B_6 at the concentrations ranging from 10^<-7>M to 10^<-5>M in the minimum medium of Davis and Mingioli supplemented with glucose. The bacterium, however, utilized the phosphate forms in the phosphate starving medium of Garen and Levinthal which is known to derepress alkaline phosphatase, and it grew well on 10^<-6>M pyridoxine phosphate, 10^<-6>M pyridoxal phosphate or 10^<-5>M pyridoxamine phosphate. Correspondingly, the bacterial cells grown in the minimum medium could not take up the phosphate esters of tritium labelled vitamin B_6 compounds at 10^<-6>M concentrations in the same medium, whereas the cells grown in the phosphate starving medium significantly took up the labelled compounds in the same medium. The main labelled vitamin B_6 compound in the phosphate starving medium after 30-min incubation of labelled pyridoxine phosphate with the cells grown in the same medium was found to be pyridoxine. Furthermore, uptake of the labelled pyridoxine phosphate by E. coli K 12 cells was essentially similar to that by E. coli KG 980 cells. These results indicate that the phosphate forms of vitamin B_6 can be taken up and utilized after de phosphorylation by alkaline phosphatase, but not taken up in their intact form.

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When male albino rats were fed with a vitamin B_1-deficient diet designed by Gubler, ^<1)> the contents of vitamin B_6 and vitamin B_<12> in the blood after 3 weeks feeding decreased to 1/2 and 1/3 those of the pair-fed animals, respectively. Daily oral administration of insufficient amounts of vitamin B_1 to the vitamin B_1-deficient animals maintained their lives for 8 days or so. Daily supplement of vitamin B_6 and vitamin B_<12> to the B_1-insufficient level could elongate the survival of the vitamin B_1-deficient rate more than 20 days. These facts suggest that there would exist some interrelationships among vitamin B_1,B_6 and B_<12> in their blood contents and physiological functions in the animal.

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穀類・豆類・疏菜の種子に含まれタカジアスターゼ処理又は酸加水分解によって遊離してくるB_6量をSaccharomyces carlsbergensisを用いた微生物法により比較し, 米糠に次いでミツバ種子に高レベルのB_6誘導体の含まれることを認めた.米糠に種々の加水分解酵素を作用させてβ-グルコシダーゼに高いB_6遊離能のあることから, "PIN-β-グルコシド"の存在を推定した.更にセファデックスG-10ゲル濾過によって分別した画分の酸加水分解, タカジアスターゼ処理, β-グルコシダーゼ処理によって"PIN-β-グルコシド"以外にも低分子のタカジアスターゼ易消化性及び難消化性化合物の存在を認めた.

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