In tree squirrels, nests have important functions as places for breeding, sleeping, hiding, resting, and social behavior. However, few quantitative studies have been performed on the various functions of nests in the field. In the present study, we built a nest box equipped with a video camera in the field, and quantified the frequency of each behavior in nest use of Japanese giant flying squirrels (Petaurista leucogenys). Three nest boxes were set in a forest in Hachioji City, Tokyo, and a total of 3,549 days were recorded on video from February 2010 to May 2014 using the motion detection function. The percentage of days the flying squirrel used the nest boxes was 33.8%. The total number of visits was 2,114 times. Although flying squirrels used the nest box for roosting (639 times) and raising young (207 times) in daytime, they also frequently used it at night; for example, peeping (238 times), patrols (283 times), dropping in (260 times), sleeping (294 times), and raising young (193 times). The nest use at night was suggested to have three functions : exploration, rest, and breeding. Flying squirrels use the nest frequently for various purposes throughout the year, not only during the day but also at night.
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