巻号一覧
64 巻, 2 号
選択された号の論文の23件中1～23を表示しています
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VIII. Ocurrence of Ceramide Mono- and Dihexoside in Corbicula, corbicula sandaiTARO HORI, OSAMU ITASAKA, MICHIKO KAMIMURA1968 年 64 巻 2 号 p. 125-128
発行日: 1968/08/25
公開日: 2008/06/30
ジャーナル フリーThe isolation and characterization of glycolipids (already reported as Fr. 1 (1)) from the tissues of corbicula, Corbicula sandai, are described. Each glycolipid was purified by column chromatography on Florisil, cleaved by methanolic HCl and the components were determined mainly by gas chromatography.
Ceramide monohexosides consisted of glucosyl-ceramide (77%) and galactosyl-ceramide (23%). The ceramide dihexoside was different from any glycolipids of higher animals in its mannose content in place of galactose. The tentative structure was proposed to be mannosylglucosyl-ceramide.抄録全体を表示PDF形式でダウンロード (241K) -
KOKI YOKOTSUKA, MICHITERU YOSHIDA, KENSUKE SHIMURA1968 年 64 巻 2 号 p. 129-136
発行日: 1968/08/25
公開日: 2008/06/30
ジャーナル フリー1. A countercurrent distribution method was applied to the fractionation and purification of histones. By the distribution with water and butanol-2 solvent systems containing various concentrations of urea and trichloroacetic acid, slightly lysine-rich histones from calf thymus and posterior silkglands were further fractionated into seven and six components, respectively, and an arginine-rich histone from calf thymus into nine components.
2. Although no significant differences were found in the distribution patterns of slightly lysine-rich histones from calf thymus and posterior silkglands, obvious differences in amino acid composition and N-terminal amino acids between these two histones were observed.
3. Of the fractions obtained by the distribution, AC1 and AC3 fractions from an arginine-rich histone appeared to be the most homogeneous on gel electrophoresis and N-terminal amino acid analysis.
4. Thus, the countercurrent distribution method has proved to be an excellent tool for purification of histones, and has been suggested to be applicable to other histone fractions, if we choose solvent systems of suitable distribution-coefficient values by changing the concentrations of urea and trichloroacetic acid in the solvent system of water and butanol-2.抄録全体を表示PDF形式でダウンロード (1101K) -
II. Intermediate Formation of Phosphoryl ProteinTAIBO YAMAMOTO, YUJI TONOMURA1968 年 64 巻 2 号 p. 137-145
発行日: 1968/08/25
公開日: 2008/06/30
ジャーナル フリーWe have previously reported the dependencies on the concentration of Ca++ and ATP of the Ca++-dependent ATPase [EC 3. 6. 1. 3] activity of sarcoplasmic reticulum from rabbit skeletal muscle. We have also observed the 32P-incorporation into the enzyme, when it was incubated with γ-32P-ATP in the presence of Ca++. The P incorporation increased with increase in the concentration of Ca++. Furthermore, the rate of Ca++-dependent ATPase activity was proportional to the amount of Pinconorated. In the present work, we studied the mechanism of the 82P-incorporation and obtained the following results.
1. The amount of P incorporated increased with increase in pH and reached a maximum above pH 8.3. The pH-activity curve of the Ca++-dependent ATPase was bell-shaped with a maximum at pH 7.0. The pH dependence of the ratio of the ATPase activity to the amount of 32P-incorporation indicated the participation of a functional group with a pK value of about 7.2 in the decomposition of the enzymephosphate complex.
2. When 2mM EGTA was added after formation of the enzymephosphate complex, the amount of the complex decreased rapidly. The rate of its decomposition decreased with increase in pH value. The life-time of the complex measured in this way agreed well with the value obtained kinetically, i.e., from the ratio of the ATPase activity to the amount of the complex.
3. When 2mM of cold ATP were added after the formation of the enzyme-32P complex, the amount of 32P incorporated decreased with time. The rate of its decrease was 3 fold that observed after the addition of EGTA.
4. The rate of decomposition of the enzyme-phosphate complex was found to be unaffected by removal of magnesium ion which was required for the formation of the enzyme-phosphate complex.
5. The enzyme-phosphate complex isolated by TCA-treatment was stable in acidic medium but unstable in alkaline medium. The complex was very unstable in the presence of 0.5M hydroxylamine.抄録全体を表示PDF形式でダウンロード (576K) -
Spectral Interactions of Protoheme with Ethyl Isocyanide and Some Other Ligands in Neutral pH RegionYOSHIO IMAI, RYO SATO1968 年 64 巻 2 号 p. 147-159
発行日: 1968/08/25
公開日: 2008/06/30
ジャーナル フリー1. At neutral pH values, dithionite-reduced protoheme interacted with ethyl isocyanide to form three spectrally different compounds. From the dependence of the spectrum on the ethyl isocyanide concentration, the products having Soret peaks at 414 and 428 mμ were assigned to the mono-isocyanide compound and the normal di-isocyanide compound (“n” form), respectively. The third species (“a” form) was anomalous in that it showed a Soret peak at 455 mμ.
2. Various experimental conditions affected profoundly the intensities of the peaks at 428 and 455 mμ in the presence of a saturating concentration of ethyl isocyanide. Thus, decrease in pH, increase in protoheme concentration, and increase in ionic strength all caused increased formation of the a form, accompanied by corresponding decrease in the amount of the n form. On the other hand, addition of organic solvents, such as phenol and aliphatic alcohols, and of deoxycholate shifted the equilibrium in favor of the formation of the n form. The maximum extent of the mono-isocyanide compound formation was also affected by pH; its formation was increased as the medium became more alkaline.
3. Circular dichroism measurements indicated that the a form, but not the n form, was optically asymmetric.
4. Pyridine, another lipophilic ligand, also showed anomalous behavior, similar to that of ethyl isocyanide, on interaction with protoheme in the neutral pH region. However, no such unusual interactions were observed between protoheme and hydrophilic ligands such as cyanide and imidazole.
5. From these observations, it was suggested that the a form of the protoheme compound with ethyl isocyanide or any other lipophilic ligand represented aggregated states of the protoheme compound carrying two moles of ligand attached to the ferrous iron. The formation of such aggregates seemed to be favored by the high hydrophobicity of the protoheme compound.
6. The spectral behavior of the protoheme-ethyl isocyanide system was similar in many respects to that of the ethyl isocyanide compound of the reduced form of P-450. The state of protoheme in this microsomal hemoprotein is discussed based on these similarities.抄録全体を表示PDF形式でダウンロード (983K) -
FUMIO SAWADA, FUMIE ISHII1968 年 64 巻 2 号 p. 161-165
発行日: 1968/08/25
公開日: 2008/06/30
ジャーナル フリー1. The interaction between bovine pancreatic ribonuclease [EC 2. 7 .7. 16] (RNase) and 4-thiouridine 2'(3')-phosphate was compared with that between RNase and uridine 2'(3')-phosphate.
2. The difference spectrum of the RNase-4-thiouridylic acid complex was similar in shape to that of the RNase-uridylic acid complex, but was at about 70 mμ longer wavelengths.
3. The pH dependence of the spectral change (hypochromism) of the two complexes were similar, the maxima being at between pH 5 and 6.
4. The dissociation constants of the both complexes were estimated by spectrophotometric titration to be 1.1×10-5M at pH 5. 6, 0.01 ionic strength and 25°C.
5. Gel filtration showed that both nucleotides bind to the enzyme in a one to one ratio.
6. These results indicate that the modes of interaction of the two nucleotides with RNase are similar.抄録全体を表示PDF形式でダウンロード (288K) -
MASAHIKO KODAMA, YUSAKU TAGASHIRA, CHIKAYOSHI NAGATA1968 年 64 巻 2 号 p. 167-170
発行日: 1968/08/25
公開日: 2008/06/30
ジャーナル フリーBase specificity of pinacyanol which has been shown to interact with DNA orienting perpendicularly to the base planes, was studied by means of the difference spectrum method. Purine bases in DNA were found to be more preferable than pyrimidine bases for the attachment site of pinacyanol. The same was true for the nucleosides.
The base specificity of carcinogenic 4-nitroquinoline-1-oxide (4-NQO) and 4-hydroxyaminoquinoline 1-oxide (4-HAQO) was studied, and in this case also, purine bases were more effective in binding with these carcinogens. There was no difference in binding capacity between adenosine and guanosine.抄録全体を表示PDF形式でダウンロード (299K) -
KYOKO TORII, YASUYUKI OGURA1968 年 64 巻 2 号 p. 171-179
発行日: 1968/08/25
公開日: 2008/06/30
ジャーナル フリー1. The EPR spectra of horse erythrocyte catalase [EC 1. 11. 1. 6] and its derivatives were measured at liquid nitrogen temperature. Free catalase and HF-catalase gave EPR spectra having two intense signals with g-values of 6.6 and 5.4 at low magnetic field and a weak signal with g-value of 2.03 at high magnetic field. The EPR spectrum of HCN-catalase showed three signals with g-values of 1.66, 2.25 and 2.84, and that of HN3-catalase exhibited signals with g-values of 6.7, 5.2, 2.80, 2.18 and 1.74. From the data, the electronic states of the hematinirons of free catalase and HF-catalase were of a high-spin type, and those of HCN-catalase and HN3-catalase were of low-spin type and of a mixture of high- and low-spin types, respectively.
2. The optical absorption spectra of free catalase and the HF- and HCN-complexes measured at liquid nitrogen temperature were essentially the same as those obtained at room temperature. However, the optical absorption spectrum of HN3-catalase at liquid nitrogen temperature was of a mixture of high- and low-spin types, although the absorption spectrum of this complex at room temperature was of high-spin type. The absorption maxima, which are characteristic of the low-spin type, appeared at temperatures below −80°C; that is, in HN3-catalase, there seemed to be thermal equilibrium between the low- and high-spin forms.
3. The energy differences between the ζ and η or ζ orbitals of the HCN- and HN3-complexes were calculated from the EPR signals of the low-spin type. It was found that the energy difference between the ζ and η orbitals of dε is 900cm-1 for HCN-catalase and 1000cm-1 for HN3-catalase, and the difference between the ζ and ζ orbitals of dε is 2000cm-1 for HCN-catalase and 2700cm-1 for HN3-catalase. From the data on the EPR spectra of the high-spin type, the energy difference, Ey−Ex, was found to be 600cm-1 for free catalase and HF-catalase, and 700cm-1 for HN3-catalase.抄録全体を表示PDF形式でダウンロード (631K) -
II. Properties of Leucyl-, and Tyrosyl-tRNA SynthetasesYOSHITO KAZIRO, YASUSHI TAKAHASHI, NORIKO INOUE1968 年 64 巻 2 号 p. 181-188
発行日: 1968/08/25
公開日: 2008/06/30
ジャーナル フリーThe properties of leucyl- and tyrosyl-tRNA synthetases [EC 6. 1. 1. 4 and 6. 1. 1. 1] from Pseudomonas aeruginosa are described. Both enzymes are stable when kept frozen at -20°C in the presence of 25% glycerol. The purified preparation are slightly contaminated with inactive proteins as judged by polyacrylamide gel electrophoresis. The kinetic constants of the two enzymes are determined using ATP_??_32PPi exchange assay and 14C-amino-acyl-tRNA synthesis assay. Both enzymes display a rather broad pH optimum ranging from 7.5 to 9.0 in Tris-HCl buffer.
Leucyl-tRNA synthetase requires the presence of NH4+ ions for its activity. The stimulatory effect of NH4+ ions is found to be on the second step of the leucyl-tRNA synthetase reaction, namely the transfer of the leucyl group from an enzyme-bound leucyl adenylate to tRNA, for the rate of ATP _??_32PPi exchange is not affected by NH4+ ions.
Leucyl-, and tyrosyl-tRNA synthetases of P. aeruginosa aminoacylate E. coli tRNA to the same extent as the homologous E. coli enzymes, but do not react with yeast tRNA. Purified leucyl-tRNA synthetase of P. aeruginosa is able to recognize the multiple E. coli tRNA species specific for leucine obtained by fractionation on a DEAE-sephadex column.抄録全体を表示PDF形式でダウンロード (1064K) -
TAKAO NAKAMURA, NOBUO MAKINO, YASUYUKI OGURA1968 年 64 巻 2 号 p. 189-195
発行日: 1968/08/25
公開日: 2008/06/30
ジャーナル フリーAscorbate oxidase [EC 1. 10. 3. 3] was purified from cucumber, Cucumis sativus, and its molecular weight and other physicochemical properties were investigated. This enzyme contains 8 atoms of copper per molecular weight of 132, 000 and has a specific activity of 3, 500 Dawson's units/mg. Results on spectrophotometric and ESR measurements, as well as those on kinetic analysis and azide inhibition of the enzyme, are also presented.抄録全体を表示PDF形式でダウンロード (1199K) -
KYOZO OGURA, TOKUZO NISHINO, SHUICHI SETO1968 年 64 巻 2 号 p. 197-203
発行日: 1968/08/25
公開日: 2008/06/30
ジャーナル フリーPrenyltransferase (farnesyl pyrophosphate synthetase) [EC 2. 5. 1. 1] and isopentenylpyrophosphate isomerase [EC 5. 3. 3. 2] were obtained and partially purified from pumpkin fruit. The prenyltransferase preparation catalyzed the condensation of isopentenyl pyrophosphate with dimethylallyl pyrophosphate as well as with geranyl pyrophosphate to yield trans-traps farnesyl pyrophosphate as a final product, and was free of isopentenyl pyrophosphate isomerase and geranylgeranyl pyrophosphate synthetase activities. Prenyltransferase of pumpkin has properties similar to those of pig liver, showing requirement of Mg++, Km value of 1.3×10-6M for geranyl pyrophosphate, and pH optimum at 7.5.抄録全体を表示PDF形式でダウンロード (518K) -
XVI. Occurrence of Ceramide Pentasaccharide in the Membrane of Erythrocytes and Reticulocytes of RabbitTOMOKO ETO, YOICHI ICHIKAWA, KENJI NISHIMURA, SUSUMU ANDO, TAMIO YAMAK ...1968 年 64 巻 2 号 p. 205-213
発行日: 1968/08/25
公開日: 2008/06/30
ジャーナル フリーGlycolipids were obtained from rabbit erythrocytes and reticulocytes. A new glycolipid, galactosyl-(1→3)-galactosyl-(1→_3)-N-acetylglucosaminyl-(1→3)-galactosyl-(1→4)-glucosyl ceramide, was found as the main component of erythrocyte glycolipids. Galactosyl-(1→4)-galactosyl-(1→4)-glucosyl ceramide and a small amount of dihexosyl ceramide were also found.
Unlike mature erythrocyte glycolipids, those of reticulocytes contain much mono- and di-hexosyl ceramide and an unknown glycolipid in addition to trihexosyl ceramide and ceramide pentasaccharide, suggesting that these glycolipids are metabolized actively in immature cells.
The structure of the carbohydrate moiety in the ceramide pentasaccharide is very similar to that of the blood group B active mucoid from body fluids and the glycolipid inhibited hemagglutination of human B-erythrocytes by the corresponding iso-antibody.抄録全体を表示PDF形式でダウンロード (596K) -
XVIII. An Improved Method for Purification of the Proteinase_??_b from the Venom of Agkistrodon halys blomhoffii and Its Physicochemical PropertiesGENICHIRO OSHIMA, SADAAKI IWANAGA, TOMOJI SUZUKI1968 年 64 巻 2 号 p. 215-225
発行日: 1968/08/25
公開日: 2008/06/30
ジャーナル フリー1. Lots of proteinase b, one of the two hemorrhagic proteins in the venom of Agkistrodon halys blomhoffii, were prepared by the modified methods using a column chromatography on DEAE-cellulose and a gel filtration on Sephadex G-100. The preparation obtained was homogeneous in ultracentrifugation, in free boundary electrophoresis and on column chromatographies.
2. Sedimentation and diffusion constants of proteinase b were 5.54 S, and 5.26×10-7cm2/sec, respectively, and its molecular weight was found to be about 95, 000. Its isoelectric point was pH 4.18. The mobility at pH 8.51 for the descending pattern was -5.82× 10-5cm2/sec volt.
3. The content of aspartic acid was 12.5 per cent and this value was higher than those of usual proteins. Chemical compositions of proteinase b were as follows: nitrogen 12.4 per cent, polypeptide moiety 76.5 per cent, neutral sugars (galactose, mannose and trace of fucose) 8 per cent, glucosamine 6.5 per cent, and sialic acid 3 per cent. Thus, the venom proteinase b was characterized to be a glycoprotein.抄録全体を表示PDF形式でダウンロード (1854K) -
XIX. Purification and Some Physicochemical Properties of Proteinases a and c from the Venom of Agkistrodon halys blomhoffiiGENICHIRO OSHIMA, YUSHI MATSUO, SADAAKI IWANAGA, TOMOJI SUZUKI1968 年 64 巻 2 号 p. 227-238
発行日: 1968/08/25
公開日: 2008/06/30
ジャーナル フリー1. Proteinases a and c from the venom of A. halys blomhoffii have been purified by successive use of ion-exchange chromatography and gel filtration. From 32.7g of the lyophilized venom 138mg of purified proteinase a and 2.69g of purified proteinase c were obtained. The purified preparations of proteinases a and c are essentially homogeneous as observed by sedimentation in the ultracentrifuge, free-boundary electrophoresis and column chromatography.
2. The values of the sedimentation constants extrapolated to zero concentration, s_??_, w, are 3.63 S and 4.94 S for proteinase a and c, respectively. The diffusion constant for proteinase c, D_??_, w, is 5.88×10-7cm2 sec-1. The partial specific volume of proteinase c, _??_, is 0.695ml/g and its intrinsic viscosity, [η], is 0.046 dl/g. The molecular weights of proteinases a and c are computed to be about 50, 000 for the former and about 70, 000 for the latter. Free-boundary electrophoreses give isoelectric points of pH 6.0 for proteinase a and 3.85 for proteinase c. Their absorbancies A1%1cm at 280mμ, are 9.08 and 10.98 for proteinases a and c, respectively. Their contents of nitrogen are 13.83 and 13.90 per cent and those of polypeptides 76.5 and 91.2 per cent for proteinases a and c, respectively.
3. Both proteinases a and c contain sugars in their molecules, 13.6 per cent for the former and 8.46 per cent for the latter.抄録全体を表示PDF形式でダウンロード (1884K) -
I. Differential Iodination of Tyrosine ResiduesKATSUYA HAYASHI, TADAHISA SHIMODA, KATSUHIKO YAMADA, AKIHIKO KUMAI, MA ...1968 年 64 巻 2 号 p. 239-245
発行日: 1968/08/25
公開日: 2008/06/30
ジャーナル フリーThe differential iodination of tyrosine residues in the lysozyme [EC 3. 2. 1. 17] molecule was investigated in consideration of their positions in the three dimensional structure of lysozyme crystal. One tyrosine residue was unreactive on iodination, while other two residues were stepwise iodinated. Under appropriate conditions, the two residues were iodinated completely to diiodotyrosine. The fractionation of iodinated lysozyme with CM-cellulose failed because of the deiodination of iodotyrosine by the adsorbent.
From the measurement of the optical density at 320mμ and spectrophotometric titration of iodinated lysozyme, it was concluded that the iodination controlled under appropriate conditions proceeded stepwise and homogeneously.抄録全体を表示PDF形式でダウンロード (454K) -
HIROYA KAWASAKI, NOBUO OJIMA, HISAKO TADA, HITOSHI SATO1968 年 64 巻 2 号 p. 247-250
発行日: 1968/08/25
公開日: 2008/06/30
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HIROH IKEZAWA, TOSHIKO SEGI, OTOHARU ISHIZAKA1968 年 64 巻 2 号 p. 251-253
発行日: 1968/08/25
公開日: 2008/06/30
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YASUO OGAWA1968 年 64 巻 2 号 p. 255-257
発行日: 1968/08/25
公開日: 2008/06/30
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HAJIME ITOH, MICHIYUKI YAMADA, SHIRO TOMINO, KIYOSHI KURAHASHI1968 年 64 巻 2 号 p. 259-261
発行日: 1968/08/25
公開日: 2008/06/30
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KOSCAK MARUYAMA, MASARU KAWAMURA1968 年 64 巻 2 号 p. 263-265
発行日: 1968/08/25
公開日: 2008/06/30
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TAKAO NAKAMURA, YASUYUKI OGURA1968 年 64 巻 2 号 p. 267-270
発行日: 1968/08/25
公開日: 2008/06/30
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KOUR TAKAHASHI, KOICHI YAGI1968 年 64 巻 2 号 p. 271-273
発行日: 1968/08/25
公開日: 2008/06/30
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OSAMU MINARI, HIROKO TSUBONO, MASAKO AKIYAMA, TOSHIO SAKAGAMI1968 年 64 巻 2 号 p. 275-278
発行日: 1968/08/25
公開日: 2008/06/30
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GENJI MATSUDA, TETSUO MAITA, HIROSHI TAKEI, HISAHIRO OTA, MASATO YAMAG ...1968 年 64 巻 2 号 p. 279-282
発行日: 1968/08/25
公開日: 2008/06/30
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