Using the suspension of rabbit or chick blood cells, the concentrative uptake of thiamine and thiamine alkyl disulfides by the cells was ob-served.
1. Neither thiamine, thiamine disulfide nor thiamine pyrophos-phate was taken up by the cells at all. The addition of glucose or adenosine triphosphate did not favour the uptake of them.
2. Thiamine alkyl disulfides tested were all readily taken up by the blood cells, the rate of uptake depending upon temperature, concentration gradient and pH in the medium. From the effect of pH on the dissociation and partition coefficient, only the unionized form of thiamine methyl disulfide seemed to be transferred by the simple diffusion process.
3. The partition coefficients of the thiamine alkyl disulfide homolo-gues beared logarithmically a linear relationship to the mumber of carbon atoms in the alkyl side chain. Among the homologues the rate of penetration depends upon the partition coefficient.
4. From all these results, it was concluded that, unlike thiamine, thiamine alkyl disulfides were taken up by the blood cells according to the process of solution in, and diffusion through, the lipid membrane.
Acknowledgment. All samples of thiamine derivatives were kindly offered by Dr. Matsukawa. The technical assistance of Miss M. Kurihara is gratefully acknowledged. The authors are deeply indebted to Mr. Asahi for measuring pK, and Mr. Terao for measuring the solubility. The authers' thanks are also due to Dr. Kuwada, Director of our Research Laboratory and to Dr. Ninomiya, our previous chief, for their continued encouragements.
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