The Journal of Biochemistry
Online ISSN : 1756-2651
Print ISSN : 0021-924X
40 巻, 1 号
選択された号の論文の9件中1~9を表示しています
  • MASAO UCHIDA, KATASHI MAKINO
    1953 年40 巻1 号 p. 1-5
    発行日: 1953年
    公開日: 2008/11/18
    ジャーナル フリー
    The authors determined active hydrogens of guanosine and yeast nucleic acid by the method proposed by Roger J. Williams which consists in dissolving the substances in deuterium oxide, evaporating to dryness and determining the increase in, weight due to the replacement of active hydrogen by deuterium. The experiment showed that the number of active hydrogen of guanosine was 6, corresponding to the theoretical number, while the tetrasodium salt of the yeast nucleic acid was found to have 11-12 active hydrogens.
    Acknowledgment. We are greatly indebted to Prof. Titani for the kind supply of deuterium oxide. The work was aided by a grant from the Scientific Research Fund of the Department of Education.
  • IV. DISTRIBUTION OF LIPID-HEXOSAMINE AND LIPID-HEMATAMINIC ACID IN THE RED BLOOD CORPUSCLES OF VARIOUS SPECIES OF ANIMALS
    TAMIO YAMAKAWA, SHIZUE SUZUKI
    1953 年40 巻1 号 p. 7-10
    発行日: 1953年
    公開日: 2008/11/18
    ジャーナル フリー
  • SUZUOKI ZIRÔ, SUZUOKI TUNEKO
    1953 年40 巻1 号 p. 11-20
    発行日: 1953年
    公開日: 2008/11/18
    ジャーナル フリー
    Using the suspension of rabbit or chick blood cells, the concentrative uptake of thiamine and thiamine alkyl disulfides by the cells was ob-served.
    1. Neither thiamine, thiamine disulfide nor thiamine pyrophos-phate was taken up by the cells at all. The addition of glucose or adenosine triphosphate did not favour the uptake of them.
    2. Thiamine alkyl disulfides tested were all readily taken up by the blood cells, the rate of uptake depending upon temperature, concentration gradient and pH in the medium. From the effect of pH on the dissociation and partition coefficient, only the unionized form of thiamine methyl disulfide seemed to be transferred by the simple diffusion process.
    3. The partition coefficients of the thiamine alkyl disulfide homolo-gues beared logarithmically a linear relationship to the mumber of carbon atoms in the alkyl side chain. Among the homologues the rate of penetration depends upon the partition coefficient.
    4. From all these results, it was concluded that, unlike thiamine, thiamine alkyl disulfides were taken up by the blood cells according to the process of solution in, and diffusion through, the lipid membrane.
    Acknowledgment. All samples of thiamine derivatives were kindly offered by Dr. Matsukawa. The technical assistance of Miss M. Kurihara is gratefully acknowledged. The authors are deeply indebted to Mr. Asahi for measuring pK, and Mr. Terao for measuring the solubility. The authers' thanks are also due to Dr. Kuwada, Director of our Research Laboratory and to Dr. Ninomiya, our previous chief, for their continued encouragements.
  • SHOYO SAKAGUCHI
    1953 年40 巻1 号 p. 21-25
    発行日: 1953年
    公開日: 2008/11/18
    ジャーナル フリー
    1. Nearly all sorts of protein give arginine value about one third to that of its hydrolysate. This means that two third of arginine mole-cules in protein enters into combination of its guanidine group.
    2. The guanidine linkage of arginine in protein molecule is easily splitted by mild treatment of acid.
    3. The arginine molecule bound in peptide chain can give the full colour reaction, if its guanidine group is free.
    In conclusion the author wishes to express my hearty thanks to Prof. Emeer. S. Kakiuchi and Prof. K. Kodama for their kind advice.
  • I. INTERACTIONS BETWEEN ACTOMYOSIN AND ADENOSINE TRIPHOSPHATE
    YUJI TONOMURA, SHIZUO WATANABE, KOICHI YAGI
    1953 年40 巻1 号 p. 27-54
    発行日: 1953年
    公開日: 2008/11/18
    ジャーナル フリー
    With respect to the ATP splitting and the light-scattering change upon the addition of ATP to actomyosin solution, the relationship between the velocity of these two phenomena and the ATP concen-tration, in company with the function of inorganic pyrophosphate and some cations, were analysed kinetically and then the mechanism of the two phenomena was proposed.
    It is also suggested that the ATP attacking points of actomyosin in the two phenomena are identical with each other and that the number of the active centers per one myosin molecule is six.
    The mechanism proposed is able to illustrate the various facts ob-served about muscular contraction.
    Finally, we are much indebted to Dr. Juro Horiuchi, the Head of the Research Institute for Catalysis, Prof. Hiroshi Tamiya, of Botanical Department, Faculty of Science (Tokyo University) and Prof. Naomoto Takasugi, of Chemical Department, Faculty of Science, for the facilities they have provided for this work and to Messrs Kin-jiro Sukegawa and Junshiro Makino for valuable technical assistance.
  • MASAO UCHIDA, KATASHI MAKINO
    1953 年40 巻1 号 p. 55-68
    発行日: 1953年
    公開日: 2008/11/18
    ジャーナル フリー
    1. The writer proposed the method for determination of guanine which consists of precipitation of purine with ammoniacal silver nitrate and titrätion with hypoiodite according to Willstätter and Schudel.
    2. From the analytical values of nitrogen, phosphorus, amino-nitrogen (Van Slyke's method), purine-nitrogen (copper bisulfite method) and guanine, the writer suggests that the individual bases (adenine, guanine, cytosine and uracil) in yeast nucleic acid (Merck) occur in nearly equal molar ratios.
    The present work was aided by a grant from the Scientific Research Fund of the Department of Education.
  • KYUTARO SHIMIZU
    1953 年40 巻1 号 p. 69-73
    発行日: 1953年
    公開日: 2008/11/18
    ジャーナル フリー
    The bile salts of hog were examined and a small amount of litho-cholic acid was obtained together with hyodesoxycholic acid, 3-hydroxy-6-keto-allocholanic acid and chenodesoxycholic acid.
  • KYUTARO SHIMIZU
    1953 年40 巻1 号 p. 75-79
    発行日: 1953年
    公開日: 2008/11/18
    ジャーナル フリー
    3(β), 6(β)-Dihydroxy-allocholanic acid was prepared from hyode-soxycholic acid and succinylated at the C3-hydroxyl group. 3(β)-Hydroxy-Δ5-allocholenic acid was obtained from this succinylated compound by dehydrating the C3-hydroxyl group with POCl3 and hydrolysing the dehydration product with potassium hydroxide.
  • KYUTARO SHIMIZU
    1953 年40 巻1 号 p. 81-83
    発行日: 1953年
    公開日: 2008/11/18
    ジャーナル フリー
    Cholic acid was oxidized with potassium permanganate in a bsffersolution and 3, 12-dihydroxy-7-ketocholanic acid was obtained in good yield.
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