Human albumin fractions prepared from normal, insulin resistant schizophrenic, obese, diabetic and insulin resistant diabetic subjects by Debro's procedures were found to inhibit the action of insulin on rat diaphragm muscle in vitro.
The inhibitory percentages by albumin fractions are followed, 21% in the normal, 37% in insulin resistant schizophrenia, 53% in obesity, 56% in diabetes and 74% in insulin resistant diabetes.
The inhibitory activity of albumin fractions dimi, nished after treatment with p-Chloromercuriben, zoate (albumin fractions were incubated with PCMB in distilled water, dialysed against distilled water and lyophilized to dryness).Electrophoretic and immuno-electrophoretic patterns for these albumin fractions showed a single band on cellulose acetate membrane.
The degree of absorbancy at numbers of wave 1, 000-1, 100in infra-red ray relates closely with the value of polysaccharides bound to albumin.
The contents of polysaccharides bound to albumin fractions are follow ed, 0.07mg in insulin resistant schizophrenia, 0.068mg in insulin resistant diabetes, 0.045mg in obesity, 0.04mg in diabetes and 0.03mg in the normal (the contents of polysaccharides are showed per mg of nitrogen).
The immuno-reactive insulin levels in albumin fractions are less than 7μu/ml of 2% albumin solution.
A possibility that the reactive SH groups in the albumin fractions may have an inhibitory effect in vitro and that the inhibitory activity may correlate with polysaccharides bound to albumin fractions in vivo is presented in the present report.