The strain showed a wide tolerance to salinity; that is, the optimum salinity was shown to be between 1.5 and 10%. Glucose supported good cell growth and carotenoids yield. A high content of astaxanthin was obtained from the culture on glucose, galactose, fructose, oleic acid, bean oil and sesame oil. Using yeast extracts, polypeptone, arginine acid, citruline, glycine, proline and alanine as nitrogen sources, a high total content of carotenoids was obtained. On the other hand, using nitrate and urea as nitrogen sources, a high total content of canthaxanthin was obtained.The optimum astaxanthin was at NaCl 3-4% and illumination of blue LEDs. Altermia grazed Thraustochytrium CHN-1, and was enriched with DHA and astaxanthin.Thraustochytrium CHN-1 utilized okara as a substrate for growth, and okara contained DHA and astaxanthin.