Tissue engineering is a study to construct fully functional tissues and organs using cell and scaffolds. Various tissue constructions have been attempted in vitro, such as skin, cartilage and cornea. In this study, we propose a method to construct an highly orientated muscle on chip. Furthermore, we have examined the difference of the differentiation procedure among different patterns by quantifying gene expression. PCR results show that microchannel patterning promotes myoD and myogenin expression. Combined with immunostaining results, we conclude that the optimum channel width for pattering muscle cells may be 100μm.