A microfluidic device that achieves manipulating a single cell in a simple harmonic motion up to 130 Hz is presented in this paper. Such a high-speed manipulation becomes possible because of an on-chip actuation transmitter. The transmitter plays the role of transmitting the actuation from a macro-scale actuator to a micro-scale driving force to manipulate single cells. The working principle of the transmitter is explained by a simplified mechanical model, and the experiments were conducted. Both the theoretical and experimental results show a clear enhanced dynamic response of cell manipulation with the proposed transmitter. Moreover, a pressure sensor is employed for measuring the change of fluid pressure during the manipulation for more detailed insights. The high-speed manipulation could benefit applications in the fields or biomedical diagnosis and bio-assembler.