In this study, nitrogen and phosphorus were removed by using denitrifying phosphate-accumulating organisms (DPAOs). An anaerobic / anoxic sequencing batch reactor (A₂SBR) was operated to culture the DPAOs. A₂SBR system achieved 77% of nitrogen and 83% of phosphorus removal efficiency in a system feeding actual sewage. The result showed that this system is suitable for the nutrient removal of sewage treatment. The A₂SBR sample was then subjected to microbial community analysis using FISH method and the result showed that a stable treatment process was performed at 15% or more of PAOs ratio. Additionally, a hydroxyapatite precipitation (HAP) reactor was applied as the downstream system. As a result 29% of phosphorus was recovered from the sewage by the calcium-added physicochemical technology. This HAP reactor became applicable with concentration of the phosphorus around ten-fold, although influent phosphorus was as low as about 4 mg-P/L.