Abstract
Properties of amino acid incorporation into proteins by cell-free preparations from Pasteurella multocida were examined and the following results were obtained.
1) Amino acid incorporation into proteins by the above system was dependent upon the presence of supernatant fluid, ribosomes, ATP and ATP generators, GTP and magnesium ion, and prevented by ribonuclease or chloramphenicol.
2) Amino acid incorporation into proteins by this system was stimulated by deoxyribonuclease.
3) Attachment of C14-leucine to soluble RNA was found.
4) Transfer of C14-leucine from C14-leucyl s-RNA to ribosomes was not found.
5) Incorporation of C14-leucine or C14-phenylalanine into proteins was stimulated by ribonucleic acid isolated from P. multocida or polyuridylic acid, respectively.
