Antibody titers and tuberculin skin reaction were compared in 2 groups of guinea pigs. One group of animals were injected with heat killed tubercle bacilli while animals of the second group received the bacilli in the form of Freund's adjuvant.
Changes in antibody titers of serum and skin extract were followed by Middlebrook-Dubos hemagglutination and hemolysis during the period of 10 weeks post immunization. Test for incomplete antibody was made by the Sindo-Utahashi's method.
Titers in animals injected with the adjuvant rose earlier and reached higher values than those in animals injected with tubercle bacilli alone. Generally speaking, a parallel relation was found among hemolysis, presence of incomplete antibody and tuberculin reaction as was reported earlier by Hosoda.