Recently, as a way to relieve stress and fatigue, relaxation methods such as color therapy and color healing have been used. A problem with ways of relaxing the body and mind through color use is the lack of scientific proof and their effectiveness. In this experiment, paper of four different colors(red, blue, black, white)were shown to six subjects for 10 minutes while their skin temperatures, electroencephalogram(EEG), ECG, respiration and heart rates were observed. Significantly different changes in the skin temperatures were recognized for all four colors. For the EEG, the θ, α, and β wave frequencies, amplitudes, times and places of appearance with different characteristics were observed for each color.