We have discussed about a novel technique of developing a nanostructured polypyrrole biosensor for measurement of very low concentrations of hydrogen peroxide in liquid media. The proposed fabrication method is very effective in growing a nanostructured conductive polymer layer on a planar conductive substrate. In addition enzyme loading was done under a high electric field of 1 kV/m. The developed sensor provides a linear range of 0-200 µmol/l of hydrogen peroxide and a measurement sensitivity of 6.5 Acm^-2 (mol/l)^-1.