In the present study, the stapes was examined histologically using serial sections of the temporal bone from 60 ears in 40 cases of Japanese cadavers including those of newborns to elderly persons up to age 84. As reported conventionally, the stapedial footplate showed a two-layer structure consisting of the bone layer on the tympanic cavity side and the cartilage layer on the vestibular side. The cartilage layer and the bony layer was separated basically in a linear manner. The bone and cartilage ratio in the footplate was 1: 1 in most of the cases, although some differences were seen according to age. In the borderline area between the bone and the cartilage in the footplate, no apparent progression of ossification was seen with an increase in age. Observations were also made of the sections for the presence of fissula ante fenestram (FAF). The incidence of its presence, the site of opening, difference between right and left sides, size and cell constitution within it were examined. FAF was found in 56 out of 60 ears (93.3%). In 44 ears, FAF opened on the vesibular side. As to cell constitution within FAF, typical hyaline cartilage was observed in some parts regardless of age. However, ossified fatty changes were seen in ears from elderly persons. Although this site is known as the preference site of otosclerosis, it was observed in none of the preparations examined.
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