This study evaluated the effectiveness of MHN-02, liquid food combined with whey peptides (whey protein hydrolysate), on the increase in serum interleukin (IL)-6 level after intestinal ischemia/reperfusion. Male Sprague-Dawley rats were underwent superior mesenteric artery occlusion for 45 min followed by reperfusion for 1 hr under urethane anesthesia. Test substance was administered into the duodenum 1 hr before the induction of ischemia. Serum IL-6 level was measured in course of time by ELISA. In the control rats, serum IL-6 level significantly increased in course of time after ischemia/reperfusion as compared with sham-operated rats. MHN-02 significantly inhibited the increase in serum IL-6 level as compared with the control rats. The above results show that MHN-02 may be a useful liquid food for hypercytokinaemia in the patients with severe gut ischemic shock.

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 The purpose of this study was to evaluate a factor of an antimutagenic activity of Lactococcus lactis subsp. lactis J525-2. A fermented milk prepared with this strain showed antimutagenic activity against food-borne mutagens such as 3-amino-1,4-dimethyl-5H-pyrido indole (Trp-P-1), 2-amino-6-methyldipyrido imidazole (Glu-P-1), and 2-amino-3,4-dimethyl-imidazo quinoline (MeIQ). Cultures of strain J525-2 showed high antimutagenic activity, but cell free culture showed little activity. These results suggest that extracellular products of strain J525-2 have no antimutagenic activity, and the cells play major part of the antimutagenic activity. The binding of Trp-P-1 to cells of strain J525-2 was confirmed. Mutagen binding occurred instantaneously and reached maximum at 10 min incubation. Prolongation of incubation time (up to 50 min) had no influence on the binding. The binding ability was strongly affected by pH, and the highest percentage binding was noted at pH 6.0. Cell of Lactococcus lactis subsp. lactis J525-2 was suggested to play a major role in the antimutagenic activity, and the cell could bind food-borne mutagens.

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