The parents and progenies of the cross between Haruyutaka and Katepwa were used to investigate the relationships between highmolecular-weight glutenin (HMWG) subunits and bread-making quality. The HMWG compositions of Haruyutaka and Katepwa were 1, 17+18, 2+12 and 2, 7+9, 5+10, respectively.
Glu-A1 and
Glu-B1 subunits did not show any significant effects on any of the bread-making quality indices. The
Glu-D1 subunits did not show any significant differences with the amount of flour protein, Farinograph development time or weakness. However, the
Glu-D1 5+10 subunit gave greater values of SDS-sedimentation volume (P<0.01), breaking force (P<0.001) and specific loaf volume (P<0.01) than the 2+12 subunit. On the other hand, lines with the
Glu-D1 2+12 subunit gave greater breaking deformation (P<0.001) than those with the
Glu-D1 5+10 subunit. The specific loaf volume correlated significantly with the breaking force (r=0.716, P<0.001) and the breaking deformation (r=-0.703, P<0.001). The lines giving both higher breaking force and smaller breaking deformation tended to have a high specific loaf volume. Haruyutaka gave values equivalent to lines with the
Glu-D1 2+12 subunit in breaking deformation, but the breaking force was similar to that of lines with 5+10 subunit. It could give a good estimation of bread-making quality of Haruyutaka in Japanese cultivars. The protein component besides HMWG may be associated with bread-making quality of Haruyutaka.
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