Relationship between serum levels of carotenoids and percentages of lymphocyte subsets in peripheral blood of the healthy 134 male adults, aged from 20-59 y was investigated .It was demonstrated that serum β-carotene levels were negatively associated with the peripheral blood percentages of T3 and T9 of T cell subsets, while there was no significant correlation between serum β-carotene levels and the peripheral blood percentages of T cell subsets (T3, T4, T8, T9 and activated T cells), NK cell subsets (NKH1, Leu11 and Leu7) and B cell subset (B1) in the non-smokers. In contrast, serum zeaxanthin/lutein levels were positively and significantly associated with the peripheral blood percentages of activated NK cell subsets (Leu11 and NKH1) in both all subjects and the non-smokers. So, it seemed that the healthy adults with high serum zeaxanthin/lutein levels could keep to be present high distribution of activated NK cells in peripheral blood.