Twenty mg of glucose per tube was sufficient to reach a maximal growth after the incubation for 18 hours, when varied amounts of glucose were added to MaciasR's broth with 33.6 mμg thiamine-HCl. Glucose could be replaced by maltose or fructose but the growth by lactose was inferior to that by glucose. Glucose could not be substituted by xylose but xylose with glucose stimulated the growth better than glucose alone. When glucose was present in the broth, ribose was better but lyxose or arabinose was weaker than xylose in stimulating the growth of lactobacilli.