Vitamin A depleted rats were divided into two groups (group A and B). Aqueous dispersions of β-carotene were injected twice intravenously. The quantities of carotene injected to group B was 1/2 of that to group A. The excretion and distribution of carotene administered were investigated. The carotene contents in the feces varied day by day, and no certain inclination was found. The excretion of carotene after the second injection was almost the same as that after the first injection. The rate of carotene content in the liver and feces of the rats in group B, injected with carotene, was much lower than that in group A, and thus it became obvious that the quantities of carotene injected influenced the metabolism of carotene. The tendency was surmised that the excretion of carotene into the feces decreased, as the state of vitamin A deficiency of the rats became severer.