We studied whether a statement similar to the Ghouila-Houri's theorem might hold for alternating orientations of cocomparability graphs. In this paper, we give the negative answer. We prove that it is NP-complete to decide whether a cocomparability graph has an orientation that is alternating and acyclic. Hence, cocomparability graphs with an acyclic alternating orientation form a proper subclass of alternately orientable cocomparability graphs. We also provide a separating example, that is, an alternately orientable cocomparability graph such that no alternating orientation is acyclic.