Male albino rats were devided into three groups and fed respectively on three kinds of laboratory fodders;standard diet, high sucrose diet (50% sucrose in caloric) and high fat diet (50% lard in caloric). Following the feeding on these special diets for 8-28 days, 50 mg per kg body weight of alloxan was injected subcutaneously four times, and 75 mg per kg body weight of alloxan twice every week or two weeks.
The rats in these three groups were fed on isocaloric diet through all period of this experiment. 1) Diabetes was not found in the standard diet group (9 rats).
2) Seven of the high fat diet group (12 rats) became diabetic, and ketonuria was proved in three of these seven diabetic rats.
3) Three of the high sucrose diet group (13 rats) became diabetic, ketonuria was not found in these three rats.
Although there was no diabetic rat in the standard diet group, severe diabetes was found in 58% of rats in the high fat diet group.
Therefore it was considered that the diabetogenic effect of alloxan was intensified with the high fat diat.