Glycosylated ProteinのRadioimmunoassayの基礎的検討

橋本 文代; 佐藤 光代; 大星 千鶴子; 弘田 明成; 大島 一洋; 大江 泰雄; 申 貞均; 島 健二

doi:10.11213/tonyobyo1958.30.437

Development of a Radioimmunoassay for Glycosylated Protein and Its Evaluation for Clinical Application

Fumiyo Hashimoto, Mitsuyo Sato, Chizuko Ohboshi, Meisei Hirota, Ichiyo Oshima, Yasuo Ohe, Sadaharu Shin, Kenji Shima

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Keywords: Glycosylated albumin, HbA₁c, RIA

JOURNAL FREE ACCESS

1987 Volume 30 Issue 5 Pages 437-444

DOI https://doi.org/10.11213/tonyobyo1958.30.437

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Abstract

A radioimmunoassay for glycosylated protein (GP) was developed by using an antibody to recuced glycosylated low density lipoprotein which was capable of identifying glucitol lysine epitopes on all reduced GPs studied, including human and bovine albumin. Pretreatment to eliminate free glucose and labile GP from a sample is necessary before measurement of GP in the plasma sample. The mean serum GP value of patients with IDDM (n=32) was 6.06±1.89 n mole of reduced glycosylated lysine equiv. per mg of protein, which was significantly higher than that of normal controls (n=50), 0.824±0.209 n mole of reduced glycosylated lysine euquiv. per mg of protein. The serum GP value in the patients was significantly correlated with their HbA₁c level (r=-0.56, p<0.005). The method seems to be promising for further clinical application.

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