The DPN check^® is a device that can easily perform nerve conduction studies (NCS) of the sural nerve. We assessed the validity of the DPN check^® in comparison to conventional NCS for evaluating diabetic neuropathy. We compared the measured values and determination of the presence or absence of neuropathy in 136 patients with diabetes mellitus who were evaluated by both the DPN check^® and an NCS. The correlation coefficients between the results of the DPN check^® and NCS for SNAP Amp and SCV were 0.71 and 0.60, respectively. The concordance rate for judging whether or not the severity of neuropathy is more than moderate was 82.4 % (vs. Baba's DN Classification, κ value 0.61). The DPN check^® normal group tended to have a prolonged distal latency of the median nerve (4.17 ms). It is difficult to assess the full range of neurological disorders using the DPN check^®. However, we consider it to have sufficient ability for application in screening for diabetic neuropathy.