2019 Volume 54 Issue 3 Pages 128-138
Following the description in the preceding paper of the mathematical model to calculate the detoxification of ozone by ascorbic acid in the apoplast (cell wall), we describe in this paper our implementation of the model using Microsoft Excel. While we collected various parameters of the model from the literature, little information was available about the resistance (ρ3) to inword ozone transport at plasmalemma (cell membrane). Therefore, we assumed two cases; i.e., “ρ3=0” and “ρ3 ≠ 0” in the calculation of the ozone detoxification. The ozone concentration ([O3]) at the plasmalemma has often been assumed to be zero, which is equivalent to the assumption, “ρ3=0”. In this case, ascorbate (ASC-) in the apoplast can detoxify ozone by 35.2%, whereas the remaining 64.8% of the ozone reaches the cytoplasmic membrane and cytosol to react with biological molecules causing subsequent damage. By comparison, the assumption, “ρ3≠0”, leads to a higher detoxification rate, e.g., 64.5% in the case of ρ3=80.18 s m-1 and ambient [O3] of 100 ppb. The detoxification rate increases as the ambient [O3] declines, which is consistent with the common notion of a threshold concentration in the ozone damages to plant tissues.