The demand for environmentally friendly fiber processing and cleaning formulations prompted us to study the heat resistance, solvent resistance, pH characteristics, and the activation of peroxidases from various sources. Peroxidase has a broad substrate specificity. In a wide pH range, peroxidase was found to react with various hydrogen donor. The rate of reaction with hydrogen donors were found to be influenced by the oxidation potential of the hydrogen donor. Peroxidases from different sources were found to exhibit different stabilities and reaction characteristics. Rice full peroxidase showed excellent resistance towards heat, organic solvent, and hydrogen peroxide. The reaction mechanisms of horseradish peroxidase and rice full peroxidase were studied. Since the peroxidase reaction can be controlled by the reaction conditions, these conditions were proposed to suit the specific application.