Overexpression of the laccase gene, lcc1, in Lentinula edodes using the pChG vector

Toshitsugu Sato; Yuumi Suzuki; Masayoshi Naito; Aika Minami; Naoyuki Suzuki; Kaori Yaegashi; Tatsuya Hirano

doi:10.1016/j.myc.2019.02.004

Short communication

Overexpression of the laccase gene, lcc1, in Lentinula edodes using the pChG vector

Toshitsugu Sato , Yuumi Suzuki, Masayoshi Naito, Aika Minami, Naoyuki Suzuki, Kaori Yaegashi, Tatsuya Hirano

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Keywords: Shiitake mushroom, Genetic engineering, Polyphenol oxidase, House-keeping gene promoter

JOURNAL FREE ACCESS

2019 Volume 60 Issue 4 Pages 246-249

DOI https://doi.org/10.1016/j.myc.2019.02.004

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Abstract

Lentinula edodes secretes laccase (Lcc: EC 1.10.3.2), an industrially useful enzyme. In this study, we introduced and expressed the L. edodes Lcc gene, lcc1, driven by L. edodes glyceraldehyde-3-phosphate dehydrogenase gene promoter into L.edodes. The resulting transformants showed 2-fold Lcc activity than that of the host strain, and expression of the recombinant lcc1 was confirmed by RT-PCR.

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