A simple and efficient transformation system for the edible mushroom Pleurotus eryngii

Ying Wang; Rong Wang; Wen Jun Mao; Ming Gong; Ying Nv Gao; Li Hua Tang; Rui Fang Yang; Yan Li; Chen Li Zhou; Da Peng Bao

doi:10.1016/j.myc.2016.06.005

Short communication

A simple and efficient transformation system for the edible mushroom Pleurotus eryngii

Ying Wang, Rong Wang, Wen Jun Mao, Ming Gong, Ying Nv Gao, Li Hua Tang, Rui Fang Yang, Yan Li, Chen Li Zhou, Da Peng Bao

Author information

Ying Wang
Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences
Rong Wang
Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences
Wen Jun Mao
Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences
Ming Gong
Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences
Ying Nv Gao
Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences
Li Hua Tang
Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences
Rui Fang Yang
Institute of Crop Breeding and Cultivation Research, Shanghai Academy of Agricultural Sciences,
Yan Li
Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences
Chen Li Zhou
Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences
Da Peng Bao Corresponding author
Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences

Keywords: Agrobacterium tumefaciens-mediated transformation, Glufosinate-ammonium resistance marker, Hsp70 promoter

JOURNAL FREE ACCESS

2016 Volume 57 Issue 5 Pages 356-360

DOI https://doi.org/10.1016/j.myc.2016.06.005

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Abstract

Although Pleurotus eryngii is a highly-valued mushroom, the tools for undertaking molecular biological research are lacking. We have now developed an improved, more efficient ATMT (Agrobacterium tumefaciens-mediated transformation) system for generating P. eryngii transformants. Successful generation of transformants required that nutrient-limited fungal mycelium be used as the receptor material. The transformants remained genetically stable and produced normal clamp connections in mating tests. The procedures developed here provide an effective and simple means for gene function studies and directed breeding in P. eryngii essential for realizing the full biotechnological potential of this mushroom.

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