A total of 28 deuteromycetous isolates obtained from forest environments in the Ogasawara (Bonin) Islands and the Ryukyu Islands, Japan, were identified to five Cylindrocladium and related fungal species (Calonectria kyotoensis (anamorph: Cylindrocladium floridanum), Cylindrocladiella lageniformis, Cylindrocladium camelliae, Cylindrocladium citri, and Cylindrocladium tenue), excluding two unknowns. Cylindrocladiella lageniformis is a new record, and the others are rarely reported in Japan.