フェムト秒レーザー励起パルスの波形整形による2光子励起蛍光顕微計測の高性能化

山崎 智高; 多田 潤二; 河野 太貴; 神成 文彦; 須田 亮; 緑川 克美; 河野 弘幸; 水野 秀昭; 宮脇 敦史

doi:10.2184/lsj.34.833

Highly Functional Two-Photon Excited Fluorescence Microscopy with Pulse Shaping of Femtosecond Excitation Pulses

Tomotaka YAMAZAKI, Jyunji TADA, Taiki KONO, Fumihiko KANNARI, Akira SUDA, Katumi MIDORIKAWA, Hiroyuki KAWANO, Hideaki MIZUNO, Atushi MIYAWAKI

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Keywords: Pulse shaping, Two-photon excited microscopy, Super-continuum

JOURNAL FREE ACCESS

2006 Volume 34 Issue 12 Pages 833-837

DOI https://doi.org/10.2184/lsj.34.833

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Abstract

Femtosecond laser pulses used as a pumping pulse in two-photon excited fluorescence microscopy were adaptively shaped by two separate self-learning schemes. First, the laser pulse was delivered through a singlemode fiber, and its pulse s ape was adaptively optimized before the fiber propagation so that its fluorescent efficiency reaches the highest level. Second, the laser pulse was delivered through a microstructure fiber, and the spectrum shape of the super-continuum generated by the microstructure fiber was adaptively optimized by shaping the input pulse so that the intensity ratio of fluorescence generated from a protein mixture becomes the highest.

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