The purpose of this study was to clarify the effect that playing volleyball has on the prevention of osteoporosis in women. We measured the physique and calcaneal bone density of 183 married volleyball players（48.5 ± 10.2 yrs） and 261 ordinary women（69.8 ± 8.1 yrs）. Bone density was measured by ultrasound using osteo-sono assessment. The volleyball playing period of the married volleyball players was 27.3 ± 10.8 years. Standing height, body weight and bone density of married volleyball players was higher than that of ordinary women. A positive correlation was confirmed between the relative value of bone density and body mass index in married volleyball players. The long competition period of the married volleyball players probably contributes to an increase in their calcaneal bone density. It appears that long-term volleyball playing tends to be effective for the prevention of osteoporosis in Japanese women.