The purpose of this study was to investigate the long-term effects of recombinant human basic fibroblast growth factor (rhbFGF) in the infant human alveolar process of the maxilla-derived osteoblastic cells(HAB).
The results were as follows,
1. bFGF (2.5ng/ml) significantly increased the DNA content of the HAB.
2. bFGF significantly decreased the alkaline phosphatase activity (ALP) of the HAB.
3. While bFGF inhibited the expression of ALP mRNA, and stimulated the expression of osteocalcin mRNA in HAB, it did not markedly effect the expression of type I collagen mRNA in HAB.
4. bFGF markedly inhibited the mineralized nodule formation and calcium incorporation.
These findings indicate that long-term treatment with rhbFGF stimulates cell growth, but its effect on the differentiation varies depending on each differentiation parameter in the infant human alveolar process of the maxilla-derived osteoblastic cells (HAB).