The aim of this study was to investigate whether the area covered by the acquired pellicle could be distinguished by scanning electron microscope (SEM) observation from the uncovered area and the remineralized area of the surface enamel.
The enamel surface was polished with emery paper (-#1,500) and divided into four areas. A quarter of the surface covered by plastic tape (Scotch^TM) was subjected to the control. The remainder of the surface was etched with 65% phosphoric acid for 30 sec. and the second quarter of the surface was also covered by plastic tape. The remaining half of the surface was exposed for 2 hrs to the supernatant of the centrifuged saliva to get the acquired pellicle.