The prevalence of dental caries is low in younger children, while rapid progression occurs in adolescence. The purpose of this study was to clarify the association of prevalence of dental caries in children and daily lifestyle habits. A total of 100 children (57 boys, 43 girls, average age 4 years 6 months) who visited our department were enrolled and divided into the caries and non-caries group. Each parent or guardian was asked regarding the daily lifestyle of the child, including nutrition and oral health. We found that "maternal employment", "bedtime", and "frequent snacks" were correlated with dental caries prevalence. There was no significant differences in regard to "living with grandparents","presence of brothers and/or sisters", "wake-up time", and "knowledge of dental health". Furthermore, we found that all children with caries ate excessively during meals, excluding snacks. Dental health promotion contributes to specifically target dental caries, while establishment of healthy lifestyle habits in children, particularly healthy habits for eating, is important.