Much information like depth of invasion was required owing to the advance of therapy for oral cancer. However, the handling of the surgical material as a base of the pathological examination still is not standardized. In this paper, our sampling method for Breadloaf step sectioning (BLSS) was described in order to standardize of the pathological observation of the tongue cancer. In our pathological report, the size of the cancer (pT), atypical epithelia of the surgical incisional margin (dys), the depth of invasion (depth) and the relation of the depth of invasion and the extent to the lateral side (ie) were added to mandatory procedure. Furthermore, the cooperation of both the oral surgeon and the oral pathologist in order to utilize BLSS was described.