The International Society for Clinical Electrophysiology of Vision (ISCEV) published the first standardized basic protocol in 1989 so that comparable electroretinograms (ERGs) could be recorded throughout the world. The latest version of the ISCEV ERG protocol was approved at the annual ISCEV meeting in 2008 and documented in 2009. While this protocol describes a procedure for human ERGs, it is often applied to animal ERGs. In order to help researchers and technicians easily understand this latest protocol, the present article reviews a summary of ISCEV ERG protocol, and different points between 2004's version and the latest one. Additionally, consideration when the protocol is applied to animal ERGs is described here.