2024 Volume 80 Issue 25 Article ID: 24-25022
Although the accuracy of environmental DNA (eDNA) detection is influenced by biological and environmental conditions, eDNA dynamics in turbid water is not well understood, precluding establishment of eDNA surveys in turbid water area. In this study, we evaluated the effect of different turbidity conditions on aqueous eDNA fate with different states. Water samples under five turbidity conditions (0, 20, 50, 100, and 200 ppm) were designed by mixing riverbed sediment and either distilled water or aquarium water rearing common carps (Cyprinus carpio). We filtered the turbid water samples using two types of glass fiber filters with different pore sizes and quantified carp eDNA using digital PCR. Consequently, as turbidity increased, the concentration of eDNA also increased; however, under high turbidity conditions, the eDNA concentration tended to decrease. These findings suggest that eDNA recovery is higher at moderate levels of turbidity owing to the adsorption of eDNA and the influence of PCR inhibitors.