We investigated the prevalence and characterization of antibiotic-resistant Escherichia coli in dent corn fields using sewage-based fertilizers in Tsuruoka City, Yamagata, Japan. Over a period of four months, between June and September 2023, 21 soil samples were collected in the fields that utilized composted sewage sludges and liquid centrate from sludge dewatering as sewage-based fertilizers. For comparison, an additional 27 soil samples were collected in the fields using pig manure, chemical fertilizers, and no fertilizer. The findings of quantifying the amount of E. coli in the samples detected in 48 soil samples in the early growing stage and after harvesting at the concentration ranging from 3.0×10¹ to 1.4 ×10³ CFU/g. The detection rate and concentration were not significantly affected by the application of sewage-based fertilizers. Antibiotic susceptibility tests using 18 antibiotics demon-strated that 15 (31.2%) of the 48 isolates were resistant to ciprofloxacin (13.2%), amikacin (13.2%), and ampicillin (7.5%). To identify the host origin of E. coli, phylogenetic groups analysis and MLST analysis were performed. The main phylogenetic groups were classified A (15 isolates) and B1 (15 isolates) which were normally detected in livestock hosts. The 15 isolates were classified as ST720, which has been reported in environmental isolates. These findings indicate that the identified E. coli did not come from sewage sludge sources, but rather from external environmental factors, such as surrounding fields and wildlife.