Unlike commute trips, business trips are generated throughout business hours, and several destinations tend to be visited in a trip chain. In this study, a route and departure time choice model for a business trip chain is developed based on the hypothesis that the trip chain is made so as to minimize the total cost, including costs associated with other business activities before and after the trip chain, as well as costs associated with travel time, toll fees, and the probability of being late. The empirical analysis is carried out using a data set obtained from a survey of business trip drivers. The properties of the estimated model are examined on a sample case