Mitochondria and intracytoplasmic membrane system of the yeast-like cells of Candida albicans were demonstrated by electron microscopy.
Fixation of the materials was done in a 2% aqueous solution of potassium permanganate for 48 hours at room temperature and for embedding was used a methacrylate mixture.
The mitochondria with cristae mitochondriales were surrounded by a continuous membrane and its profiles were surrounded by a continuous membrane and its profiles were circular or ellipsoidal. The cristae seemed to be formed by infolding the inner part of the limiting membrane.
The intracytoplasmic membrane system or the endoplasmic reticulum appeared to be closely connected with not only the cytoplasmic membrane but also the nuclear membrane.
These membrane systems consisted of three layers, at least; two electron dense layers and a less dense interspace. Each one was measured 35-50A in width.