The running distance and speed of sevens rugby referees were recorded by Global Positioning System (GPS), and these data were compared to those of rugby players in order to investigate corresponding physiological load levels. Subjects were six rugby players who play in regional preliminaries of high school sevens rugby, and four referees who referee their matches. Running distance and speed during matches were recorded by GPS devices. At the same time, heart rate was recorded in order to determine exercise intensity. The results show that total running distance, mean speed, and exercise intensity were significantly greater in referees than in players. In conclusion, the physiological load of sevens rugby referees during matches is high, and it is clear that conditioning measures for referees are necessary for them to make accurate decisions during matches.