Advancements in the motion of machine tools involving a five-axis-controlled machining center (5MC) and a multi-functional turning center have helped to achieve both high speed and high precision. Especially, the simultaneous motion accuracy of three axes at very high feed speed has increased markedly. However, it has become difficult to create female threads using a tapping process due to the use of difficult-to-cut materials. Therefore, we propose a processing technology for creating female threads in difficult-to-cut materials that utilizes both a thread mill tool and helical interpolation motion based on numerical control (NC) with a machining center. Here, we achieved screw cutting using three-axis motion control helical interpolation with a machining center. We examined the chatter vibration caused by threading machining by specifying the piezoelectric dynamometer and the wireless holder that can measure acceleration in the twisting direction.