Induction of Apoptotic Cell Death in HL-60 Cells by Jacaranda Seed Oil Derived Fatty Acids

Masao Yamasaki; Chihiro Motonaga; Marino Yokoyama; Aya Ikezaki; Tomoka Kakihara; Rintaro Hayasegawa; Kaede Yamasaki; Masanobu Sakono; Yoichi Sakakibara; Masahito Suiko; Kazuo Nishiyama

doi:10.5650/jos.62.925

Biochemistry and Biotechnology

Induction of Apoptotic Cell Death in HL-60 Cells by Jacaranda Seed Oil Derived Fatty Acids

Masao Yamasaki, Chihiro Motonaga, Marino Yokoyama, Aya Ikezaki, Tomoka Kakihara, Rintaro Hayasegawa, Kaede Yamasaki, Masanobu Sakono, Yoichi Sakakibara, Masahito Suiko, Kazuo Nishiyama

Author information

Masao Yamasaki
Division of Food Science and Nutrition, Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki
Chihiro Motonaga
Division of Food Science and Nutrition, Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki
Marino Yokoyama
Division of Food Science and Nutrition, Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki
Aya Ikezaki
Division of Food Science and Nutrition, Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki
Tomoka Kakihara
Division of Food Science and Nutrition, Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki
Rintaro Hayasegawa
Division of Food Science and Nutrition, Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki
Kaede Yamasaki
Division of Food Science and Nutrition, Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki
Masanobu Sakono
Division of Food Science and Nutrition, Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki
Yoichi Sakakibara
Division of Food Science and Nutrition, Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki
Masahito Suiko
Division of Food Science and Nutrition, Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki
Kazuo Nishiyama
Division of Food Science and Nutrition, Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki

Keywords: jacaric acid, jacaranda seed oil, apoptosis, oxidative stress

JOURNAL FREE ACCESS

2013 Volume 62 Issue 11 Pages 925-932

DOI https://doi.org/10.5650/jos.62.925

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Abstract

Various fatty acids are attracting considerable interest for their anticancer effects. Among them, fatty acids containing conjugated double bonds show one of the most potent cytotoxic effects on cancer cells. Here, we focused on the cancer cell killing activity of jacaranda seed oil. The seed oil of jacaranda harvested from Miyazaki in Japan contained 30.9% cis-8, trans-10, cis-12 octadecatrienoic acid, called jacaric acid (JA). Fatty acid prepared from this oil (JFA) and JA strongly induced cell death in human leukemia HL-60 cells. On the other hand, linoleic acid and trans-10, cis-12 conjugated linoleic acid (<10 μM) did not affect cell proliferation and viability. An increase in the sub-G₁ population and internucleosomal fragmentation of DNA was observed in JA- and JFA-treated cells, indicating induction of apoptotic cell death. Finally, the cytotoxic effects of JA and JFA were completely abolished by α-tocopherol. Taken together, these data suggest that jacaranda seed oil has potent apoptotic activity in HL-60 cells through induction of oxidative stress.

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