Cotelomer type zwitterionic surfactants having multi-hexyl chains and multi-carboxylate and muti-pyridinium functions (R₆S-zR₆VPQ-wVP-yAA, w and y means the number of 4-vinylpyridine and sodium acrylate, respectively; z means number of hexyl chains by quaternization) were prepared by quaternization and hydrolyses of cotelomers, which were prepared by cotelomerization of 4-vinylpyridine and methyl acrylate in presence of hexanethiol, and examined for their surface activities. Critical micelle concentration (cmc) of zwitterionic cotelomers shifted to lower concentration with increasing number of hexyl chains. The cmcs of R₆S-zR₆VPQ-wVP-yAA with z of 2.1-3.8 were from one 110th to one 4th of those of the corresponding cationic cotelomers. Zwitterionic cotelomers gave surface tension of 40-44 mN m^-1 at the cmc. The increase of number of hexyl chains in R₆S-zR₆VPQ-wVP-2.3AA series reduced the surface activities. Vigorous shaking of the mixtures of water and chloroform solution of zwitterionic cotelomers formed oil-in-water type emulsion. Highly stable emulsion was formed by using R₆S-zR₆VPQ-wVP-2.3AA with z of 1.4-2.1 and the degrees of emulsification were kept at the level of 60-70% after 360 min of standing. Zwitterionic cotelomers gave interfacial tension of 2-4 mN m^-1 at the water-chloroform interfaces and oriented themselves easier than equimolar mixtures of cationic cotelomer and anionic cotelomer.