The purpose of this research was to characterize the in vitro early cellular behavior of osteoblast-like cells, fibroblasts, and epithelial cells on both polished and wire-type electric discharge machined titanium surfaces prepared by glow discharge treatment. XPS analyses indicated glow discharge treatment produced relatively contaminant-free surfaces and improved surface wettability dramatically. Higher levels of early cell attachment were observed on surfaces after glow discharge treatment than on those without such treatment. SEM observation of attached cells on surfaces after glow discharge treatment showed better compatibility with the surface. These results suggested glow dischage treatment was useful to obtain excellent cellular behavior on prepared titanium surfaces.