We studied sustainability of the effects of enthusiastic dietary education in elementary school carry over into junior high school by comparing the foods eaten, nutritional intake, and biomarkers of junior high school boys from schools that provided dietary education and schools that did not. Differences were observed in biomarkers such as triglycerides, cholesterol and LDL that were not present in elementary school. One explanation may be a difference in volume of luxury grocery item like ice cream eaten in elementary school that remained as a dietary habit in junior high school. This suggests the need for dietary education from junior high school as well to teach healthy food consumption behavior concerning nonessential foods that can cause lifestyle diseases.