A new thermophilic spore-forming strain KG8^T was isolated from the mud of Taslidere hot spring in Batman. Strain KG8^T was aerobe, Gram-positive, rod-shaped, motile, occurring in pairs or filamentous. Growth was observed from 35-65ºC (optimum 55ºC) and at pH 5.5-9.5 (optimum pH 7.5). It was capable of utilizing starch, growth was observed until 3% NaCl (w/v) and it was positive for nitrate reduction. On the basis of 16S rRNA gene sequence similarity, strain KG8^T was shown to be related most closely to Anoxybacillus species. Chemotaxonomic data (major isoprenoid quinone—menaquinone-7; major fatty acid—iso-C15:0 and iso-C17:0) supported the affiliation of strain KG8^T to the genus Anoxybacillus. The results of DNA-DNA hybridization, physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain KG8^T. Based on these results we propose assigning a novel subspecies of Anoxybacillus kamchatkensis, to be named Anoxybacillus kamchatkensis subsp. asaccharedens subsp. nov. with the type strain KG8^T (DSM 18475^T=CIP 109280^T).