We examined the biological effects of the newly synthesized oligopeptide derived from Emdogain^® (EMD) in Sprague-Dawley rat bone marrow (RBM) cells. Cell culture experiments were performed with RBM cells. The biological effects of the oligopeptides on RBM cells at five concentrations were determined by examining cell proliferation, cell adhesion, and chemotaxis. RBM cells were cultured in a suspension of 100 ng / mL oligopeptides and osteogenic supplements (the test group) and medium without the oligopeptides (the control group). Alkaline phosphatase (ALP) activity was measured after 7 and 14 days, and Ca deposition after 14 days. The test group showed significantly higher ALP activity and Ca deposition than the control group for each period. In conclusion, these data suggest that newly synthesized oligopeptide derived from EMD induces hard tissues formation to undifferentiated periodontal mesenchymal cells as well as RBM cells.