Crude butanol extract of syngenic spontaneous squamous cell carcinoma of WHT/Ht mice demonstrated a lymphocyte blastogenesis in mixed lymphocyte-tumor antigen culture reaction and contained tumor associated antigen. In this paper, characteristics of crude butanol extract of WHT/Ht tumor were studied. Crude butanol extract was partially purified using DEAE-Sephacel column chromatography and four peaks were obtained. These peaks were called, P I~P IV and measured in mixed Iymphocyte-tumor antigen culture reaction. P Ⅲ (0.02M phosphate buffer, pH6.6, 0.5M NaCl eluents) showed a significant reactivity and the content of protein was 10¹μg. This protein was estimated to have a molecular weight of approximately 18K daltons by SDS-polyacrylamide gel electrophoresis.

However P I and P IV had a negative reactivity in mixed lymphocyte-tumor antigen culture reaction. These results suggested that P Ⅲ had an immunogenic, and P I and P Ⅳ had a suppressogenic antigen.